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  • Similar rating EEMs with price

    2018-10-23

    Similar rating EEMs with price and efficiency detail comparison with those of SMs (as per Table 1 in [1]), when processed with the cost effectiveness formulas and conditions given in [1], resulted encouraging values of annual energy savings, cost savings and payback periods. An example of such calculation is given here to support the statistics in the main paper, i.e. Ref. [1].
    Example calculation of cost effectiveness parameters for a 2HP motor The following analysis for 2HP motor operating at 75% of full rated load illustrates how to determine the cost effectiveness of obtaining an energy-efficient versus a standard efficiency motor for the initial purchase case. The formulas are used from Ref. [11]. Similar analysis for other motors in the sample lead to the 20 values for each parameter: annual energy saving, cost saving and payback periods for replaced EEMs for SMs. Fig. 5 shows the break-up of total energy consumption by sampled SMs in terms of energy consumption by similar rating EEMs and corresponding energy savings. Fig. 6 describes that the sum of annual operational cost on an EEM and its corresponding cost savings equals the annual operational cost on similar rating SM. Finally, the data described in this article is further analyzed statistically in Ref. [1] to construct the confidence bounds for energy conservation parameters.
    Data The medium composition for a hybridoma cell line was optimized applying a simple Design of Experiment approach in shaking flasks, which was verified and provided the initial set-up for upstream processing. Based on the optimized medium, various feeding strategies with and without Cell BoostTM 6 led to a prolonged process time and a higher mAb yield. For F(ab′)2/Fab generation, buy Biotin-HPDP parameters as well as affinity resins in downstream processing were evaluated for increased fragment yield coupled with SEC-MALS analysis and flow cytometry to confirm product quality and binding ability.
    Experimental design, materials and methods
    Acknowledgments This study was supported by Ulm and Biberach joint graduate school in pharmaceutical biotechnology funded by the Baden-Württemberg State Ministry of Science, Research and Arts (KPK Pharmazeutische Biotechnologie). AIR AB83-14 was kindly donated by Kenneth Siddle (Cambridge University, UK) and A14 cells were kindly provided by Margriet Ouwens (DDZ, Düsseldorf, Germany). Thanks to Steffen Schulze, Viktoria Kramer, Josephine Frühauf, Maximilian Simm, Sven Mathias, Patricia Arlt, Lisa Hospach, Meike Bass, Manuel Regenauer and Jörg Zimmermann for technical support and scientific input. Special thanks to Maureen Spearman for critically reviewing the manuscript and providing the valuable comments.
    Data
    Experimental design, materials and methods
    First cycle of drying and watering Drying: Thirty-two blocks of each type of the soil were placed in an oven at 105°C for 24h (Fig. 7). Out of the oven (Fig. 8), eight non-stabilized blocks and eight stabilized blocks of each type of the soil were grinded. We notice that the resistance to the compression of the stabilized blocks was higher than the resistance to the compression of the blocks of non-stabilized blocks. Watering: The remaining 16 blocks, eight non-stabilized blocks and eight stabilized blocks of each type of soil, were immersed in water whose temperature is 26°C for five hours (Fig. 9). The duration of immersion must be higher than the time of full saturation of blocks. After five hours of watering, we observed the following:
    Second, third, fourth and fifth cycle of drying and watering For the second cycle, only eight stabilized blocks of each type of soil were left for experimental purposes. They go successively to the third, fourth and fifth cycle of watering and drying. We noticed that the edges of the blocks of silty and clay soil crumbled, while the blocks of lateritic soils did not suffer any damage (Fig. 12). All of the blocks were crushed and removed from the water after the fifth cycle. We noticed that the resistance to a simple compression after the five cycles is higher than the resistance to simple compression of dried blocks before immersion.